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Article|10 Dec 2014|OPEN
BcRISP1, isolated from non-heading Chinese cabbage, decreases the seed set of transgenic Arabidopsis
Tongkun Liu1,2,3 , Yu Qian1,2,3 , Weike Duan1,2,3 , Jun Ren1,2,3 , Xilin Hou1,2,3 , Ying Li,1,2,3 ,
1College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
2State Key Laboratory of Crop Genetics & Germplasm Enhancement, Ministry of Agriculture, Nanjing 210095, China
3Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, China
*Corresponding author. E-mail: yingli@njau.edu.cn

Horticulture Research 1,
Article number: 62 (2014)
doi: https://doi.org/10.1038/hortres.2014.62
Views: 728

Received: 01 Aug 2014
Revised: 01 Nov 2014
Accepted: 01 Nov 2014
Published online: 10 Dec 2014

Abstract

Mitochondria are the energy sources of plant cells and are involved in regulating cell development. Ubiquinol–cytochrome c reductase iron-sulfur protein, which is necessary for mitochondrial respiration, is a subunit of mitochondrial electron transport chain multimeric enzyme complexes. To better understand the biological function of the ubiquinol–cytochrome c reductase iron–sulfur protein, the full-length cDNA of BcRISP1 was cloned; it was found to contain 810 base pairs and encode 269 amino acids. Unusually, high expression of the BcRISP1 gene in the archesporial cell stages was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of cytoplasmic male sterile lines and maintainer lines. The seed set was affected by the overexpression of BcRISP1, and shorter siliques with lower seed sets were observed in 35S::BcRISP1 Arabidopsis plants. These characteristics may have resulted from the reduced formation of pollen and impaired pollen tube growth. qRT-PCR results revealed that in 35S::BcRISP1 plants, the expression levels of the mitochondrial respiratory chain-related genes, COX10 and RIP1, were enhanced, whereas the expression levels of QCR7 and SDH2-1 were reduced. This result implies that overexpression of BcRISP1 in transgenic Arabidopsis plants may disrupt the mitochondrial electron transport chain by affecting the expression of mitochondrial respiratory chain-related genes and therefore, reducing the seed set.