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Article|01 Jan 2020|OPEN
Divergent molecular and growth responses of young “Cabernet Sauvignon” (Vitis vinifera) plants to simple and mixed infections with Grapevine rupestris stem pitting-associated virus
M. Tobar1, N. Fiore2, A. G. Pérez-Donoso1, R. León1, I. M. Rosales1 & M. Gambardella1,
1Pontificia Universidad Católica de Chile, Facultad de Agronomía e Ingeniería Forestal, Vicuña Mackena 4860, Macul, Santiago 7820436, Chile
2Universidad de Chile, Facultad de Ciencias Agronómicas, Avenida Santa Rosa 11315, La Pintana, Santiago 8820808, Chile

Horticulture Research 7,
Article number: 20002 (2020)
doi: 10.1038/hortres.2020.2
Views: 404

Received: 06 Mar 2019
Revised: 01 Nov 2019
Accepted: 13 Nov 2019
Published online: 01 Jan 2020

Abstract

Grapevine rupestris stem pitting associated virus (GRSPaV) is one of the most widely distributed viruses; even so, little is known about its effect on Vitis vinifera. To provide new insights, the effects of single and mixed GRSPaV infections on the V. vinifera cultivar “Cabernet Sauvignon” were studied by evaluating growth parameters, such as measurements of the total plant length, the number and distance of internodes and the number of leaves per shoot. In addition, parameters relating to gas exchange, i.e., the stomatal conductance, net photosynthetic rate, internal CO2 concentration and leaf transpiration, were also assessed. All the measurements were performed in one- and two-year-old plants with a single GRSPaV infection or mixed infections of GRSPaV and Grapevine fanleaf virus (GFLV). The results show that the plant phytosanitary status did not significantly alter the growth and gas exchange parameters in one-year-old plants. However, in two-year-old plants, single GRSPaV infections increased shoot elongation, which was accompanied by the overexpression of genes associated with the gibberellic acid response pathway. The gas exchange parameters of these plants were negatively affected, despite exhibiting higher LHCII gene expression. Plants with mixed infections did not have modified growth parameters, although they presented a greater reduction in the primary photosynthetic parameters evaluated with no change in LHCII expression. The results presented here confirm the co-evolution hypothesis for V. vinifera and GRSPaV during the early stages of plant development, and they provide new evidence about the effects of GRSPaV and GFLV co-infections on the “Cabernet Sauvignon” cultivar.