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Article|08 Nov 2019|OPEN
Low temperature upregulates cwp expression and modifies alternative splicing patterns, increasing the severity of cwp-induced tomato fruit cuticular microfissures
Noam Chechanovsky1, Ran Hovav1, Rina Frenkel1, Adi Faigenboim1, Yelena Eselson1, Marina Petreikov1, Michal Moy1, Shmuel Shen1 & Arthur A. Schaffer1,
1Department of Vegetable and Field Crops, Institute of Plant Sciences, Agricultural Research Organization, Rishon LeZion, Israel

Horticulture Research 6,
Article number: 19122 (2019)
doi: 10.1038/hortres.2019.122
Views: 34

Received: 30 May 2019
Revised: 12 Aug 2019
Accepted: 12 Sep 2019
Published online: 08 Nov 2019

Abstract

The cwp (cuticular water permeability) gene controls the development of cuticular microfissuring and subsequent fruit dehydration in tomato. The gene underwent silencing in the evolution of the fleshy cultivated tomato but is expressed in the primitive wild tomato relatives. The introgression of the expressed allele from the wild S. habrochaites (cwph) into the cultivated tomato (Solanum lycopersicum) leads to the phenotype of fruit water loss during and following ripening. In this report, we show that low temperature impacts on the severity of the cuticular microfissure phenotype via a combination of effects on both expression and alternative splicing of cwph. The cwp gene, comprising four exons and three introns, undergoes post-transcriptional alternative splicing processes, leading to seven alternative transcripts that differ in reading-frame lengths. Transgenic plants expressing each of the alternative transcripts identified the longest reading frame (VAR1) as the functional splice variant. Low temperature led to a strong upregulation of cwph expression, compounded by an increase in the relative proportion of the functional VAR1 transcript, leading to increased severity of microfissuring of the cuticle. In summary, we demonstrate the molecular mechanism behind the horticultural phenomenon of the low-temperature effect on cuticular microfissures in the dehydrating tomato.