Browse Articles

Article|01 Sep 2019|OPEN
New insights into the heat responses of grape leaves via combined phosphoproteomic and acetylproteomic analyses
Guo-Tian Liu1,2,3 , Jian-Fu Jiang4 , Xin-Na Liu1,3 , Jin-Zhu Jiang1,3 , Lei Sun4 , Wei Duan1 , Rui-Min Li2 and Yi Wang1,3 , David Lecourieux5,6 , Chong-Huai Liu4 , Shao-Hua Li1,3 , Li-Jun Wang,1 ,
1Beijing Key Laboratory of Grape Science and Enology and Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China
2College of Horticulture, Northwest A&F university, Yangling 712100, China
3University of Chinese Academy of Sciences, Beijing 100093, China
4Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China
5Universite´ de Bordeaux, ISVV, Ecophysiologie et Ge´nomique Fonctionnelle de la Vigne, UMR 1287, F33140 Villenave d’Ornon, France
6INRA, ISVV, Ecophysiologie et Ge´nomique Fonctionnelle de la Vigne, UMR 1287, F-33140 Villenave d’Ornon, France
*Corresponding author. E-mail: ljwang@ibcas.ac.cn

Horticulture Research 6,
Article number: 100 (2019)
doi: https://doi.org/10.1038/s41438-019-0183-x
Views: 906

Received: 28 Jan 2019
Revised: 03 Jun 2019
Accepted: 04 Jun 2019
Published online: 01 Sep 2019

Abstract

Heat stress is a serious and widespread threat to the quality and yield of many crop species, including grape (Vitis vinifera L.), which is cultivated worldwide. Here, we conducted phosphoproteomic and acetylproteomic analyses of leaves of grape plants cultivated under four distinct temperature regimes. The phosphorylation or acetylation of a total of 1011 phosphoproteins with 1828 phosphosites and 96 acetyl proteins with 148 acetyl sites changed when plants were grown at 35 °C, 40 °C, and 45 °C in comparison with the proteome profiles of plants grown at 25 °C. The greatest number of changes was observed at the relatively high temperatures. Functional classification and enrichment analysis indicated that phosphorylation, rather than acetylation, of serine/arginine-rich splicing factors was involved in the response to high temperatures. This finding is congruent with previous observations by which alternative splicing events occurred more frequently in grapevine under high temperature. Changes in acetylation patterns were more common than changes in phosphorylation patterns in photosynthesis-related proteins at high temperatures, while heat-shock proteins were associated more with modifications involving phosphorylation than with those involving acetylation. Nineteen proteins were identified with changes associated with both phosphorylation and acetylation, which is consistent with crosstalk between these posttranslational modification types.